Pacific blue fluorophore

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Marina Blue and Pacific Blue dyes, both of which are based on the 6,8-difluoro-7-hydroxycoumarin fluorophore, exhibit bright blue fluorescence emission near 460 nm (Amine-reactive, ultraviolet light-excitable fluorophores for labeling proteins and nucleic acids—Table 1.12).Fluorochrome Chart. Masterful Panel Design Reveals Insight ... Pacific Blue DAPI FXCycle Violet Vybrant DyeCycle Violet 530/30 VL2 Pacific Green Horizon BV510 vGFP LIVE/DEAD Fixable Aqua 586/20 VL3 Horizon BV570 Qdot 605 LIVE/DEAD Fixable Yellow 615/20 VL4 Horizon BV605 Qdot 625We report a new method to quantify the affinity of small molecules for proteins. This method is based on Förster resonance energy transfer (FRET) between endogenous tryptophan (Trp) residues and the coumarin-derived fluorophore Pacific Blue (PB). Tryptophan residues are frequently found in proteins near ligand-binding sites, …Structure-guided engineering of a Pacific Blue fluorophore ligase for specific protein imaging in living cells. scientific article. Statements. instance of. scholarly article. 1 reference. stated in.Quick Guide: Antibodies Conjugated to Fluorophores. Download PDF. Bio-Rad offers a wide range of antibodies conjugated to all of the most commonly used fluorescent dyes and to our new proprietary StarBright Dyes, bright dyes specifically designed for flow cytometry. We also offer Alexa Fluor and DyLight conjugates in an array of colors, both ...eFluor 450 dye is a small-molecule fluorescent dye with spectral properties similar to Pacific Blue dye. It was developed to exhibit equal or better fluorescence intensity when compared to Horizon V500 dye or Pacific Blue dye in multicolor applications. Fluorescence intensity comparison. Human PBMCs stained with Anti-CD45RA ( clone HI100 ...Alexa Fluor 405 dye. Invitrogen Alexa Fluor 405 dye is a blue-fluorescent dye with excitation ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. Alexa Fluor 405 dye is pH-insensitive over a wide molar range, helping ensure that you get stable signals in live-cell imaging applications.(a) Blue-to-green fluorescence ratio in live HEK cells co-expressing mBlueberry2, EBFP2, mTagBFP2, Electra1, and Electra2 with EGFP via the P2A self-cleaving peptide (n = 835, 573, 1698, 1229, and ...

Super Bright 436 Dye. Invitrogen eBioscience Super Bright 436 dye is a polymer-based fluorophore excited by the violet laser (405 nm) that emits signal at 436 nm with high efficacy. The technology behind the Super Bright polymer dye family makes them useful for identifying low-abundance markers on a cell surface in flow cytometry.Minimal spectral overlap with green fluorophores. →. Lower in fluorescence than Pacific Blue™ dye, higher background in some intracellular applications.Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. 441. (in buffer): 1. (in antifade): 2. microscopy, flow cytometry, fluorescence microplate reader. Invitrogen Alexa Fluor 350 dye is a blue-fluorescent dye with moderate photostability, and excitation that matches the 350 nm laser line. The brightness and photostability of blue dyes are best suited to direct imaging of high-abundance targets. We report a new method to quantify the affinity of small molecules for proteins. This method is based on Förster resonance energy transfer (FRET) between endogenous tryptophan (Trp) residues and the coumarin-derived fluorophore Pacific Blue (PB). Tryptophan residues are frequently found in proteins near ligand-binding sites, …Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ...Alexa Fluor 405 dye. Invitrogen Alexa Fluor 405 dye is a blue-fluorescent dye with excitation ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. Alexa Fluor 405 dye is pH-insensitive over a wide molar range, helping ensure that you get stable signals in live-cell imaging applications.

Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Super Bright 436 Dye. Invitrogen eBioscience Super Bright 436 dye is a polymer-based fluorophore excited by the violet laser (405 nm) that emits signal at 436 nm with high efficacy. The technology behind the Super Bright polymer dye family makes them useful for identifying low-abundance markers on a cell surface in flow cytometry. November 5, 2020. Source: Technische Universität Dresden. Summary: Blue phosphorus, an atomically thin synthetic semiconductor, becomes metallic as soon as it is converted into a double layer ...Alexa Fluor 405 63 421 - Green 450150 10,20 34000 525 m Banana m Plum pacific O APC.H7 Alexa Fluor 350 Qdot 565 at 545 Fluor 610 Indo-I Blue Low Ca2

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Learn about the features and applications of CF® dyes, Biotium's next generation fluorescent dyes for biological research. Download the CF® dye brochure and explore the wide range of colors and conjugates available.Structure-guided engineering of a Pacific Blue fluorophore ligase for specific protein imaging in living cells 2011 Sep 27;50 (38):8221-5. doi: 10.1021/bi201037r. Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA. PMC3181222 10.1021/bi201037r Catalytic Domain / genetics512/25 VL2 Pacific Green™ LIVE/DEAD® Fixable Aqua Violet Ratiometric Probe (F2N12S) vGFP AmCyan BV510 Horizon™ V500 VioGreen™ 603/48 VL3 Pacific Orange™ Qdot® 605 LIVE/DEAD® Fixable Yellow Violet Ratiometric Probe (F2N12S) BV570 BV605 BV650 eFluor® 605NC 710/50 VL4 Qdot® 705 BV650 BV711 Blue (488 nm) 530/30 BL1 Alexa Fluor ® 488 ... 32 blue fluorophore 7-hydroxycoumarin (HC) 2 (Figure 1A, top). 33 In the course of our screening, however, we discovered several 34 structures that are not accepted by W37 point mutants. One of 35 the most interesting examples is Pacific Blue (PB), 7 a 36 fluorophore that differs from HC only in the two fluorineSep 9, 2011 · An example is the blue fluorophore Pacific Blue, which is isosteric to 7-hydroxycoumarin, and yet not recognized by the latter’s ligase (W37V LplA) or any tryptophan 37 point mutant. Here we report the results of a structure-guided, two-residue screening matrix to discover an LplA double mutant, E20G/W37T LplA, that ligates Pacific Blue as ...

Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ... Product No: C09734. CytoFLEX V5-B5-R3 Flow Cytometer has 3 active lasers (405 nm, 488 nm, 638 nm) and 13 channels for fluorescence detection. This instrument can be upgraded with a maximum of 5 Violet, 5 Blue, and 3 Red channels.The Cytek Aurora leverages full spectrum technology to provide unprecedented flexibility to scientists, enabling the use of a wide array of new fluorochrome combinations without reconfiguring the system for each application. The Aurora system delivers high-resolution data at the single-cell level to resolve the most challenging cell populations ...Are you looking for health insurance? Blue Cross insurance is one provider option that is widely available and, therefore, is likely to come up in your search. Learn more about where and how Blue Cross operates, including information on wha...eFluor 450 dye is a small-molecule fluorescent dye with spectral properties similar to Pacific Blue dye. It was developed to exhibit equal or better fluorescence intensity when compared to Horizon V500 dye or Pacific Blue dye in multicolor applications. Fluorescence intensity comparison. Human PBMCs stained with Anti-CD45RA ( clone HI100 ...Quick Guide: Antibodies Conjugated to Fluorophores. Download PDF. Bio-Rad offers a wide range of antibodies conjugated to all of the most commonly used fluorescent dyes and to our new proprietary StarBright Dyes, bright dyes specifically designed for flow cytometry. We also offer Alexa Fluor and DyLight conjugates in an array of colors, both ... Fluorophore Selection Guide. Fluorophores enable researchers to expand the limits of their multicolor experiments in high content analysis, microscopy, and flow cytometry platforms. Our fluorescent dyes are offered conjugated to antibodies, streptavidin, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling ...Pacific Green dye is a bright green-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 411/510 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 ...Pacific BlueTM 404 456 360,405,407 EviTag TM quantum dots-Lake Placid Blue 470 490 ... donor and acceptor fluorochrome molecule, placed in close proximity, allowing for energy transfer between the two. The tandem dye is excited at the excitation wavelength of the donor molecule and emits a photon atAbstract. Taxoids such as paclitaxel (Taxol) are an important class of anticancer drugs that bind β-tubulin and stabilize cellular microtubules. To provide new chemical tools for studies of microtubules, we synthesized derivatives of paclitaxel modified at the 7-position with the small coumarin-derived fluorophore Pacific Blue (PB).Pacific Blue ; Pacific Green ; Pacific Orange ; Tetramethylrhodamine (TRITC) Texas Red (and Texas Red-X) PE, APC, related tandems APC-eFluor 780 ... Alexa Fluor 700 dye is a near-IR fluorophore used primarily for flow cytometry, optimally excited by the red laser. Photostability in buffer : Dim: 633-640: 720/30: 702: 723 : Relative staining ...

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Ghost Dye™ Blue 516. Ghost Dye™ Red 710. Ghost Dye™ Red 780. Ghost Dye™ UV 450 ... Pacific Blue. Pacific Orange. PE. PE-Alexa Fluor® 610. PE-Alexa Fluor® 700. PE ...Pacific Blue (ex 410/em 455) Pacific Blue is a blue, fluorescent, coumarin based dye, excitable by the 405 nm laser. It emits at 455 nm and is best used for markers that are highly expressed due to its dim nature. This fluorophore is not compatible with StarBright Violet 440 Dye due to similar emission spectra and spillover characteristics. Filter. Mirror. Some common fluorochromes. A. V525. 525/50. 495LP. BV510, Pacific Orange, Zombie Aqua. B. V450. 450/50. —. DAPI, BV421, AF405, Pacific Blue, ...Fluorophore Selection Guide. Fluorophores enable researchers to expand the limits of their multicolor experiments in high content analysis, microscopy, and flow cytometry platforms. Our fluorescent dyes are offered conjugated to antibodies, streptavidin, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling ...Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ... For comparison, Pacific Blue (PB), a single molecule dye with near maximum absorption at 405 nm, has an extinction coefficient of 4.6 × 10 4 M −1 cm −1, a quantum efficiency of 78%, and a longer lifetime of 9 ns. Practically, the brightness of a fluorochrome is proportional to the extinction coefficient and quantum efficiency, and ...Pacific Blue® (Pac Blue, PacBlue) is a blue-emitting organic fluorophore that can be excited by the 405 nm Violet laser and captured with a 450/50 nm bandpass filter. Pacific Blue has an excititaion peak at 401 nm and an emission peak at 452 nm. Spectra Alternative Dyes to Pacific Blue Check to add to spectra viewer Pacific Blue V450 ViaFluor 405

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Cytek®’s Aurora Spectral Cytometer has a rapidly growing presence among flow cytometry research instruments. The Aurora is available with 3 or 5 laser configurations (355 nm, 405 nm, 488 nm, 561 nm and 633 nm), with the latter offering up to 64 different fluorescent parameters. This is only the theoretical limit as we continue to strive to ...PB NHS Ester. PB dye (Pacific Blue™) is a bright, blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. The excitation and emission spectrum of PB dye offers minimal overlap with green fluorophore emission spectra, making it an ideal choice for the first violet channel. Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ...Product No: C09734. CytoFLEX V5-B5-R3 Flow Cytometer has 3 active lasers (405 nm, 488 nm, 638 nm) and 13 channels for fluorescence detection. This instrument can be upgraded with a maximum of 5 Violet, 5 Blue, and 3 Red channels.The fluorophore standard beads were first run and detector gains adjusted to put the bead signals near the top third of the log scale (e.g., MFI of 1,000 on a scale of 1-1 10,000). The MFI for the standard beads in the detector channels used for FITC, PE, APC, and Pacific Blue were recorded.Pacific Blue, or systemically called 3-carboxy-6,8-difluoro-7-hydroxycoumarin, is a fluorophore used in cell biology. Its maximum excitation wavelength is 401 nm and its maximum emission wavelength is 452 nm. Even at a pH of about 7, its fluorescence is still very high. Pacific Blue is a member of the Pacific Dye Group.The Fluorophore Brightness Index Score is a relative indication of fluorescence intensity above the background for each fluorophore antibody conjugation (1=dim, 5=brightest). These values can differ depending upon the flow cytometer, the instrument filters and settings, laser power, the antibody clone and the antigen target, f/p ratios, the ...Spectrum [Pacific Blue] Pacific Blue is a fluorescent compound with an excitation peak at 404 nm and an emission peak at 455 nm. Other spectra of interest include: iFluor 405, iFluor 440, and iFluor 430. Pacific Blue belongs to the following categories: Amine Reactive Dyes and Probes for Conjugation. Purchase Pacific Blue here.SYTOX Blue Vybrant DyeCycle Violet FxCycle Violet Annexin V, Pacific Blue Annexin V, eFluor 450 PO-PRO-1 CellTrace Violet Click-iT Plus EdU Pacific Blue Azurite Cerulean TagBFP ECFP mTurquoise AmCyan BD Horizon BV421 BD Horizon V450 VioBlue 510/50 520/35 525/50 530/40 550/40 VL2 512/25 VL2(V6) 525/50 eFluor 506 Pacific Green LIVE/DEAD Fixable AquaBrilliant Violet dyes, including Brilliant Violet 421 (BV421), are a polymer dye-based technology and compatible with both spectral flow cytometry, as well as traditional flow cytometry. The violet laser has unique channels far from heavily occupied detectors allowing for larger panels. BV421 is excited by the 405 nm violet laser and emits at ... Brilliant™ Violet 510 (BV510) is a green-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 510/50 bandpass filter. BV510 has an excitation peak at 405 nm and an emission peak at 510 nm, and is similar to StarBright Violet 515 (Bio-Rad). BV510 is most often used in flow cytometry due ...... fluorophores (e.g. Pacific Blue) with highly expressed markers. Spread from a bright fluorophore will mask low level fluorescence in nearby channels. ….

eFluor 450 dye is a small-molecule fluorescent dye with spectral properties similar to Pacific Blue dye. It was developed to exhibit equal or better fluorescence intensity when compared to Horizon V500 dye or Pacific Blue dye in multicolor applications. Fluorescence intensity comparison. Human PBMCs stained with Anti-CD45RA ( clone HI100 ...Sep 9, 2011 · An example is the blue fluorophore Pacific Blue, which is isosteric to 7-hydroxycoumarin, and yet not recognized by the latter’s ligase (W37V LplA) or any tryptophan 37 point mutant. Here we report the results of a structure-guided, two-residue screening matrix to discover an LplA double mutant, E20G/W37T LplA, that ligates Pacific Blue as ... f.hubspotusercontent00.netWhen fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. ... Pacific Blue, and BD Horizon V450 cannot ...In the basic 4-color panel (BV421, FITC, PE, APC), all fluorophores are pretty bright, so you don’t need to worry too much about matching marker expression level and fluorophore brightness. Note: FITC is the dimmest of those four fluorophores, and Pacific Blue and eFluor450 can be alternatives to BV421 but they are dimmer fluorophores.Pacific Blue (ex 410/em 455) Pacific Blue is a blue, fluorescent, coumarin based dye, excitable by the 405 nm laser. It emits at 455 nm and is best used for markers that are highly expressed due to its dim nature. This fluorophore is not compatible with StarBright Violet 440 Dye due to similar emission spectra and spillover characteristics.Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ...PE-Cyanine7 dye. Fluorescence SpectraViewer. PE-Cyanine7 is a tandem fluorophore that can be excited by a broad range of lasers including 488 nm, 532 nm, and 561 nm. Dye degradation is common if exposed to photobleaching, fixation, and higher temperatures. This is a bright dye often used in flow cytometry for low-abundance antigens.Pacific Blue, or systematically 3-carboxy-6,8-difluoro-7-hydroxycoumarin, is a fluorophore used in cell biology. Its excitation maximum lies at 401 nm, while its emission maximum is at 452 nm. [2] In contrast to the less acidic 7-hydroxy-3-carboxycoumarin (pKa=7.0), the high acidity of the phenol of Pacific Blue (pKa=3.7) causes its ... Pacific blue fluorophore, [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1]