Blue pippin

In brief, size selection was performed on genomic DNA from the two affected individuals using Blue Pippin (cassette kit: BUF7510; size range: 30–40 kb). After assessment of DNA integrity and concentration, the library was prepared with a SQK-LSK108 library preparation kit and sequenced using PromethION sequencing. The data from PromethION ...

Description. DNA Size Selection for Next-Gen Sequencing, with Pulsed-Field. (100bp – 50kb) Size-Select DNA Fragments up to 50kb Collect HMW DNA with High-Pass Filtering. The BluePippin has the DNA capabilities of the Pippin Prep, plus the extra benefit of pulsed-field electrophoresis for resolving and collecting high molecular weight DNA.Seal the PCR plate with foil and centrifuge at 1000 x g for 1 minute at room t emper ature. 5. Place the sealed PCR plate into the therm ocycler. 6. Amplify the cpn60 library following the cpn60 ...Product Overview. Documents. GlycoBlue Coprecipitant consists of a blue dye covalently linked to glycogen, a branched chain carbohydrate, which is useful as a nucleic acid coprecipitant. The attached dye increases visibility of the pellet. This product is an ideal coprecipitant in nuclease protection assays at 1/100 dilution of stock solution.BEVERLY, Mass. — November 11, 2014 — Sage Science, a developer of life science products for improving sample preparation processes in molecular biology applications, today introduced the PippinHT, a high-throughput version of the popular Pippin line of automated DNA size selection instruments for use with next-generation sequencers.Library preparation and sequencing. We prepared three pooled sequencing libraries for the 438 samples following the 3RADseq protocol as described by Graham et al. (), with one modification: we size-selected for 650-850-bp fragments on a Blue Pippin (Sage Science).The concentration of each index group was determined by using a Qubit, and the average fragment length was determined by using a ...The core facility staff will wear face mask in the laboratory, which hosts multiple instruments for shared use by researchers via scheduling. Users are required to follow the University's COVID-19 Policies and Guidance on this web page: The primary contact for the Genomics Core Facility is Wei Wang, the Director, [email protected], 609-258-6998.

The reason that Pippin does not expect to find any tobacco after leaving the Shire is because the Shire is where tobacco is cultivated for smoking. No other place in Middle Earth does so, though it is found throughout the lands. ... (Capstan Blue) in two-ounce tins. That bill also carries a short note from Tolkien: "Quite correct, & I apologize ...Pippin Prep DNA Size Selection System Selects 100 bp - 1.5 kb. SageELF Whole Sample Fractionation System DNA (up to 40 kb) or Proteins (up to 500 kDa). Pippin Pulse Electrophoresis Power Supply Resolves DNA up to 400kb on a midi gel box. Applications. Transposase Enzyme Linked Long-Read Sequencing ...BluePippin users are generating longer reads from their Oxford Nanopore MinION sequencers, as demonstrated in a new app note from our colleagues at Nippon Genetics. The app note showcases a workflow used by Kazuharu Arakawa, an associate professor in the Institute for Advanced Biosciences at Keio University in Japan. The app note describes an ...the gel. The analytical gel was run with a Pippin Pulse using the 10-48kb pre-set protocol and run for 15 hours. 0.75% SeqKem Gold agarose from Lonza was used. 4 kb 5 kb 8 kb 6 kb 10 kb None (Control DNA) 6 kb 7 kb 8 kb 10 kb High-Pass threshold setting 29 kb 36 kb Marker S1 At the end of a run, marker peaks will be detected in designated ...Degraded gDNA samples (blue bars) from 2 different cell lines were processed using each of the 4 Demonstrated Protocols (red and green bars). The 40 kb cutoff protocols yield the best results. Over 60% of the recovered DNA (post-size selection) is greater than 50 kb for all 4 DNA test samples.Sage Science Blue Pippin - Gel-based size-selection of fragments from 100bp to >30kb (depending on cassette and assay). Life Technologies Qubit 3.0 Fluorometer - Fluorescence-based quantitation using intercalating dye specific for DNA, RNA, or protein (so no contamination is included). Only 1μl of sample is required. MBCL:Pippin Prep DNA Size Selection System Selects 100 bpd - 1.5 kb. SageELF Whole Specimen Fractionation System DNA (up to 40 kb) or Proteins (up toward 500 kDa). Pippin Pulses Electrophoresis Power Supply Resolves DNA up to 400kb on an midi gel box. Applications. Transposase Enzyme Linked Long-Read Sequencing ...BluePippin Operations Manual 460013 Rev D 1-1 1 Introduction Thank you for purchasing the BluePippin from Sage Science. The BluePippin is an

We’ve taken the pulsed-field power supply out of the BluePippin instrument to provide researchers an affordable tool to resolve DNA out to 100 kb and beyond. The Pippin Pulse features a simple software interface to provide users full flexibility to develop their own field-inversion protocols. The operating range is 25-175V, and up to 200 mA ...Author summary The Neisseria genus contains many genetically related commensals of animals and humans, and two human pathogens, Neisseria gonorrhoeae and Neisseria meningitidis. The mechanisms allowing commensal Neisseria to maintain a niche in their host is little understood. To identify genes required for persistence, we screened a library of transposon mutants of Neisseria musculi, a ...Auntie Mabel is knitting a blue jumper for her sister Eadie when she runs out of wool. Frogs Pippin the dog meets some frogs in her garden, and Auntie Mabel finds some frogspawn.BluePippin Operations Manual 460013 Rev B 1-2 1.2 How the System Works The BluePippin systems use pre-cast and disposable agarose gel cassettes.[PIPPIN] My days are brighter than morning air Evergreen pine and autumn blue But all my days are twice as fair If I could share My days with you My nights are warmer than firecoals Incense and stars and smoke bamboo But nights were warm beyond compare If I could share My nights with you To dance in my dreams To shine when I need the sun With youThe Genome in a Bottle Consortium, hosted by the National Institute of Standards and Technology (NIST) is creating reference materials and data for human genome sequencing, as well as methods for ...

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For PacBio libraries, we followed the study of Chakraborty et al. by shearing the DNA using a 1.5-inch 24-gauge blunt-tipped needle, and the library was prepared using PacBio's SMRTbell template protocol and Blue Pippin size selection using a 15- to 50-kb cutoff. We generated several dozen libraries in this manner and collected 124 total ...Technical note ALTERNATIVE SIZE SELECTION METHODS FOR SMRTBELL ® PREP KIT 3.0 Introduction In Procedure & checklist — Preparing whole genome and metagenome sequencing libraries using SMRTbell prep kit 3.0, size-selection beads are used to remove short DNA fragments and enrich for the long fragments that are most valuable for …Nov 5, 2020 · 最主要的是普通的切胶回收只能回收中小片段，不能回收三代平台所需的大片段。. 因此手工切胶回收正在被其他更安全简便的方法替代。. 其次是磁珠分选，磁珠分选在二代测序中应用比较广泛。. 原理是磁珠会优先抓取较大的核酸片段，在分选过程中，通过 ... Pippin Hill Farm & Vineyards 2015 Easton Blue; Pippin Hill Farm & Vineyards 2019 Cabernet Sauvignon; Pippin Hill Farm & Vineyards 2019 Chardonnay Reserve; Pippin Hill Farm & Vineyards 2019 Wild Common Reserve; Pippin Hill Farm & Vineyards 2020 Chardonnay; Pippin Hill Farm & Vineyards 2020 Reserve Rosé; Pollak Vineyards 2019 Petit Verdot ...

He added a second Pippin Prep as well as the longer-fragment Blue Pippin to his arsenal earlier this year. In Sharpe's lab, which also serves as a core facility for NRC and other Canadian government agencies, assembly projects tend to focus on large plant and fungal genomes. His team relies on Illumina and 454 sequencing, often adopting a ...The Blue Pippin system . from Sage Science enables targeted size selection. High-pass filtering kits for the Blue Pippin are beneficial for long-read sequencing, as they allow the user to collect all the sample above a specified threshold, enabling analysis of only the fragment length of interest. Size selection therefore makes it possible toThe BluePippin system uses dye-free, pre-cast gel cassettes to separate and extract desired DNA fragment size ranges from agarose. It enables size selection for fragment lengths between 90 bp to 50 kb using different gel cassettes. The BluePippin system can run pulsed-field gel electrophoresis programs and thus separate large DNA fragments. Cassette Kit Product Number Cassette Type Software DNA Control Kit Product Number BLG7510 0.75% and Capture 2 - 5 kb Marker D1 CBC7501 BLG7510 0.75% Band Capture 5 - 10 kb Marker D1 CBC760198 subscribers Subscribe 16K views 11 years ago This video demonstrates how Pippin Prep and BluePippin from Sage Science (www.sagescience.com) perform automated DNA size selection for the...The Genome in a Bottle Consortium, hosted by the National Institute of Standards and Technology (NIST) is creating reference materials and data for human genome sequencing, as well as methods for ...Following ligation of SMRTbell adapters, sheared gDNA was size selected with a 4-kb cutoff using Blue Pippin electrophoresis (Sage Science) to generate a greater fraction of long reads capable of resolving repeat sequencing in the A. xylosoxidans genome. Sequencing was performed using the PacBio RS II platform.Pippin depicted everyday events, historical figures, and religious themes using a simplified style of flat shapes and strong colors. In the American popular press of the 1910s and 1920s, the blue tiger was a recurring symbol of the unattainable, and the black bear signified the wilderness. The tiger and bear, rendered in contrasting tones, seem ...The PippinHT system is the top choice for higher-throughput workflows. The PippinHT can process 11 size selections at once on a gel cassette (1 of the 12 lanes must be dedicated to an external size marker). The PippinHT accommodates 2 gel cassettes so up to 22 samples can be run at once, and at a quarter of the run time (1 hr 15 m) when ... Upgrading Pippin Instrument Software 1. Insert the USB drive with the upgrade folder into the USB port on the front panel of the BluePippin instrument. 2. From the Main Tab, press the "INFO" button. This will launch the information window. 3. From the Information Window, press "SOFTWARE UPGRADE", press"OK" at the warning prompt.1. Blue Pippin Start up 1.1. Powering up the Blue Pippin. The Blue Pippin instrument has to be rebooted each time it is used in order to connect to the server. Once it is turned off it loses the connection. Either reboot or if the device is turned off prior to use, locate the black power button at the rear of Blue Pippin. Push the button and ...

Capable of analyzing genomic DNA (up to 60,000 bp) Has comparable assays, including high sensitivity DNA 5000 and 1000, RNA, and high sensitivity RNA. Faster run times (approximately 1 minute per sample) Lower cost. More flexibility (run 1 to 15 samples, price is scaled with the number of samples ordered) Does NOT have a small RNA assay.

Blue Pikmin, as their name implies, are a blue species of Pikmin that reside on PNF-404. Besides coloration, these Pikmin are distinguishable from other Pikmin species by their mouths, a trait …In freshwater mussels (Bivalvia: Unionida), the evolutionary conservation of DUI and unique sex-specific mitochondrial genes has led to the hypothesis that mtDNAs could play a direct role in sex determination (Breton et al. 2011; Breton et al. 2022).Unlike most other DUI lineages where F and M mtDNA divergence is often ~15% or less, the F and M mtDNAs of freshwater mussels are more than 50% ...The DNA sample was fragmented with Megaruptor (Diagenode) to 15 kbp and size-selected (>10 kbp) using the Blue Pippin (Sage Science), and prepared for sequencing using the SMRTbell Express ...Follow the links below to our Youtube page for information or training on Pippin Products: General Sage Science Product Overview, 2020 17:42 SageHLS HMW Library System User Guide. These videos are intended to help users visualize some instructions from the user manual: SageHLS YouTube playlist. Individual videos: HLS-CATCH BRCA1 Demonstration 31:40The BluePippin system uses dye-free, pre-cast gel cassettes to separate and extract desired DNA fragment size ranges from agarose. It enables size selection for fragment lengths between 90 bp to 50 kb using different gel cassettes. The BluePippin system can run pulsed-field gel electrophoresis programs and thus separate large DNA fragments. Applications. This popular instrument is a fluorescence reader for individual tubes that is used in conjunction with reagents kits to quantify dsDNA, ssDNA, RNA, and protein. This reader is easy to use and much more sensitive that the Nanodrop and is the method of choice for quantifying Illumina libraries or low concentration samples.Pippin Prep DNA Size Selection System Selects 100 bp - 1.5 kb. SageELF Whole Sample Fractionation System DNA (up to 40 kb) or Proteins (up to 500 kDa). Pippin Pulse Electrophoresis Power Supply Resolves DNA up to 400kb on a midi gel box. Applications. Transposase Enzyme Linked Long-Read Sequencing ...Fragment size may be measured using several methods (e.g., pulse-field, low percentage agarose gel analysis, blue pippin). This quality checkpoint . is important during the development and validation of the test. Labs may elect to omit this quality check after validation if the test has proven robust and stable.

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Posted on August 3, 2023 by Alex. Range+T Size Selection Sage's DNA size selection technology has been used to help improve PacBio sequencing, particularly for mammalian and complex genome work since the introduction of the BluePippin (2012). Over the last few years, PacBio®'s Hi-Fi circular consensus sequencing … more ».Size selection on the Pippin is easier, safer, and more reproducible than traditional agarose gel extraction protocols involving a UV illuminator and a razor blade. ... Fragment Analyzer traces of 5 different Blue Pippin size selected Illumina libraries. Fragments between 275 and 525 bp were targeted in each library.The BluePippin produces higher quality DNA, improving downstream analysis. Pre-cast agarose gel cassettes are constructed in a way that eliminates cross contamination. WHY use the BluePippin? (Advantages) The CGRB houses multiple Illumina and Pacific Biosciences next generation sequencing platforms that all have applications that benefit from ... The Sage Science portfolio includes products to perform DNA or cDNA sizing and protein fractionation. pippin.png. DNA Size Selection System. Pippin Prep ...Check out the latest papers citing automated DNA size selection from Sage Science. Check out our new app note with @Diagenode! Best practices when using #Megaruptor3 w/ #PippinHT or #BluePippin. Includes our new Range+T size selection method & tips on getting tight HMW DNA in the 9-30kb range. #PacBio #HiFisequencing #nanopore #genomics https ... The DNA was sheared to approximately 20kb, size separated using a Blue Pippin using the PAC-30 KB cassette (Sage Science). Libraries were prepared for PacBio sequencing using the large insert library protocol and Illumina sequencing using the TruSeq PCR-free kit per manufacturer recommendations. Long read sequencing was conducted on the Pacific ...Holothuroids, or sea cucumbers, are a diverse echinoderm group of economically significant marine benthic invertebrates (Han et al., 2016;Miller et al., 2017). Duo to its large bode size and thick body wall, stichopus monotuberculatus, a tropical holothuroid has become a popular tonic food in Indo-west pacific counties, where it is known as its delectable taste and high nutritional value (Chen ...Pipkin's Market, Montgomery, Ohio. 4,794 likes · 10 talking about this · 882 were here. Your neighborhood Farmer's Market, only better! Fresh Produce & Curated Garden CenterPippin Prep DNA Size Selection System Selects 100 bp - 1.5 kb. SageELF Whole Sample Fractionation System DNA (up to 40 kb) or Proteins (up to 500 kDa). Pippin Pulse Electrophoresis Power Supply Resolves DNA up to 400kb on a midi gel box. Applications. Transposase Enzyme Linked Long-Read Sequencing ...Following adapter ligation, samples were pooled, and these pools were size selected for a range of 430-600 bp using a Blue Pippin (Sage Science). After size selection, samples were PCR-amplified with pool-specific indexed primers, and amplification products were further pooled into a single sample based on molarity calculations from analysis ...978-744-2244 www.sagescience.com [email protected] BluePippin Operations Manual software v 6.13 cassette definition Set 15 1-2 For Size Selection of large DNA fragments (>2kb): Dye-free gels with labeled external marker - A fluorescein labeled DNA marker set is loaded in a dedicated sample lane during a run. ….

Sage Science and Blue are trademarks Pippin of Sage Science, Inc. All other brands and name mentioned herein are property of their owners. Page 3: Table Of Contents ... Figure 4.3. BluePippin front panel USB Port Indicator Light: Indicator Light: Blue when instrument is Green when electrophoresis is ready for use (power is on, underway ...Pipkin's Market, Montgomery, Ohio. 4,794 likes · 10 talking about this · 882 were here. Your neighborhood Farmer's Market, only better! Fresh Produce & Curated Garden CenterBlue Pippin Instrument Pulsed-field automated DNA size selection system The Sage Science product portfolio combines pre-cast agarose cassettes with a high-precision, automated solution for the accurate and targeted size-selection of DNA fragments for library construction in NGS workflows FlexibilityBlue Pippin Usage Form Date: Name: PI Name: Account #: Billing Contact: Billing Address: Cassettes Used (enter number): Any problems encountered during run? ITF Notes: Date Billed: Fill form: Try Risk Free. Form Popularity .Lego Lord of the Rings Merry and Pippin. In the game, Merry and Pippin's picture has a blue and green background and just wondered why. Is it just to distinguish the two. Been on my mind for years so any answer would be much appreciated. No way, this was the only post I could find about this after I came back to the game after several years and ...Double digest restriction-site associated sequencing (ddRAD-seq) is a flexible and cost-effective strategy for providing in-depth insights into the genetic architecture of germplasm collections.The larger sample wells now allow a maximum load of 10 μg (100% up from standard Blue Pippin cassettes). Also the size of the elution module has been increased as well as the surface area of the filtration membrane, bringing about improved sample recovery and reproducibility. Blue Pippin software requirement: v6.31/6.40 CD31Page 92 978-922-1832 www.sagescience.com [email protected] 500 Cummings Center Suite 2400 Beverly, MA 01915 25-15 BluePippin Operations Manual 460013 Rev F... View and Download Sage Science BluePippin operation manual online. DNA Size Selection System. Blue pippin, [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1]